Produce Michaelis-Menten and Lineweaver-Burk plots from the following laboratory data. Plot each on a separate page of graph paper (use computer-generated plots for this assignment), taking care to construct the graphs properly and using the entire full page for each graph. Construct these graphs using a computer and Excel^® to assist you.

Determine the actual K_m values from the Lineweaver-Burk plots by 1) calculating the equation for the lines (by creating a "trend line" using linear regression), and 2) setting x and then y in the equation from part 1 equal to zero to determine intercept values. Try to predict the type of inhibition shown in these graphs. Which type of plot was most useful in determining the type of inhibition, and which was better to determine the numerical values of K_m and V_max? The assignment is due at the beginning of class on Tuesday (September 15). You are to turn in:

	Michaelis-Menten and Lineweaver-Burk plots (uninhibited and inhibited reactions plotted on same graphs)
	equations for both Lineweaver-Burk plots (uninhibited and inhibited reactions)
	values of Km and Vmax for uninhibited and inhibited reactions
	determination of the type of inhibition (competitive or non-competitive)

NOTE: If you REALLY want to get the most from the time you invest in this exercise, then spend some time with the computer and once you determine K_m values, enter the calculated X-intercept into your data set and re-plot, thus forcing the line to intercept the X-axis to the left (negative) of the origin.